Choose the best answer from a-d for this question: You have two proteins, protei
ID: 1004061 • Letter: C
Question
Choose the best answer from a-d for this question: You have two proteins, protein A and protein B, that you believe interact with one another and form a quaternary structure/complex (the AB complex). You have two containers, one that holds protein A, and one that holds protein B. You mix the two containers together into a new container. Which of the following techniques would best determine if if the two proteins in your new container have complexed together? a)A typical SDS PAGE b)Size exclusion chromatography c)IR Spectroscopy d)1H NMR
Explanation / Answer
b)Size exclusion chromatography
Explanataion: by size exclusion chromatography,protein mixture will elute with different time frame w.r.t. simple protein Aand B.