Use the following information for questions 1 through 8 Most facultative anaerob

Question

Use the following information for questions 1 through 8 Most facultative anaerobic organisms that produce ethanol as a product use the glycolysis pathway followed by alcohol fermentation to produce ATP from glucose. The laboratory you work for has discovered an organism called E. hugdahlis that uses several reactions not found in the typical glycolysis pathway (reaction 1, reaction 2, and reaction 3 on the figure below). Only the three specified reactions utilize new enzymes, all other reactions utilize identical enzymes to typical glycolysis and alcohol fermentation. The new pathway is shown below: çO2 H+OH H20 20 reaction 2 reaction 1 HO-H glucose glucose-6-P reaction NAD NADH NADH HOH ATP ADP CH2OPO3 CO2 2-keto-3-deoxy 6-phosphogluconic acid H- OH CH2OPo32 reaction 3 NAD NADH CO2 acetaidehyde CO2 ethanol pyruvate NAD NADH ethanol acetaldehyde pyruvate+ - glyceraldehyde 3phosphate

Explanation / Answer

C1 and C6 of glucose should be labelled to obtain 100% methyl labelled pyruvate.

for reference : http://public.hbut.edu.cn/sgxy/swhx0/course/UploadFile/Basic%20Concepts%20in%20Biochemistry/56576_22.pdf

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