Following DNA Extraction, once an absorbance is obtained, the value is Plugged i

Question

Following DNA Extraction, once an absorbance is obtained, the value is Plugged into an equation to determine the concentration of DNA in the sample. [DNA] = (A_260 * D_f)/0.02 If you diluted your sample by a factor of 9.00 and obtained an absorbance of 0.0750, what is the DNA concentration of your original sample (in ng/mu L)? 33.75 Given the concentration found above, how much DNA (in PL) would you need to add to your PCR reaction mixture to achieve the desired amount of DNA in solution? mu L How much Sterile Water? mu L

Explanation / Answer

With a DNA concentration of 33.75 ng/uL

Desnity of DNA taken 1.4 g/ml

amount of DNA needed = 33.75 ng x 10^-9/1.4 g/ml = 2.411 x 10^-8 ml x 1000 = 2.411 x 10^-5 microliter

Amount of sterile water taken = 7.60 x 10^-5 microliter

The combination of the two solutions would give a total volume of 10 microliter

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