Please answer the following Developmental Biology question in detail: A) Below i
ID: 101975 • Letter: P
Question
Please answer the following Developmental Biology question in detail:
A) Below is a figure from a study trying to understand how the developmentally relevant snail and twisttranscription factors can facilitate progression of cancer (not migrating to migrating). In particular, Panel A shows the presence or absence of specific proteins (labels on the right/side of the images) in a vector control (no twist or snail expressed) and cells transfected to express either snail or twist (labels on the top of the figures). Based on this information, what technique was likely used for this study?
B) Using the figure above, what could you conclude about the relationship between Snail, Twist , Vimentin and E-cad?
HMLE Vector Snail Twist E-cad 0 N-cad Vim Snail Twist HMLEExplanation / Answer
A) immunohistochemistry - in this technique protein aka antigens in a tissue section are imaged by using antigen specific antibodies having a fluorescent tag.
B) in panel A 2nd row, green fluorescence is for e-cadherin which is present only when empty vector was transfected. This is supported by the blot in panel B where there is no band for e-cadherin in cases of snail and twist over-expression. This indicates that snail and twist both inhibit the expression of e-cadherins.
In the 3rd row of panel A, vimentin(green fluorescence) is absent in vector while present in the other two. From the blot we can say that vimentin has a thicker band in twist as compared to snail over-expression while no band in case of vector transfected cells. This is suggestive of the fact that snail and twist both induce the expression of vimentin with twist doing so at a higher extent than snail.