I think I have a pretty clear understanding of the steps of cloning genes into b

Question

I think I have a pretty clear understanding of the steps of cloning genes into bacteria but I'm stuck on this question. is (i) just about blotting and (ii) about nucleic acid hybridization? Please help!

When scientists clone a foreign gene into a bacterial cell, they must screen the entire population of cells to (i) identify which bacteria cells have been transformed and (ii) identify which transformed cells actually carry recombinant plasmid.
How are (i) and (ii) accomplished?
Explain briefly how each method works.

Explanation / Answer

For me the key words that I'm seeing in your question is the "foreign gene" part. If the foreign gene sequence is known, a southern blot is possible. But, if they do not know the sequence of the gene yet, blotting can't be done and you have to rely on blue/white screening as well as the antibiotic resistance conferred by a plasmid. for (ii) it would be a description of the steps that occur in the processes from (i)

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