Experiments performed with biological systems require a buffer that will not int

Question

Experiments performed with biological systems require a buffer that will not interfere with the stability and activities of the biological components. In 1966, Norman Good and his colleagues began a series of investigations that established criteria for new zwitterionic buffers that would be optimal for experimentation with biological systems. These criteria included: a. pKa between 6 and 8 b. highly water soluble c. minimally permeable to biological membranes d. minimal effects of salt concentration e. minimal effect of temperature on pKa (? pKa/°C)

Experiments performed with biological systems require a buffer that will not interfere with the stability and ap do activities of the biological components. In 1966, Norman Good and his colleagues began a series o investigations that established criteria for new zwitterionic buffers that would be optimal for experimentation with biological systems. These criteria included a. pKa between 6 and 8 b. highly water soluble c. minimally permeable to biological membranes d. minimal effects of salt concentration e. minimal effect of temperature on pKg (ApKJ°C

Explanation / Answer

Properties of acetate buffer

a) Highly water soluble: the condition meets

b) pKa / 0C = -0.0002: the condition meets, the effect of temperature is minimal (<0.02 0C)

c) pKa 4.76 : It does'nt meet, the pKa should be between 6-8: Answer

d) does not cross the bilipid layer: the condition meets as it has minimal permeability to biological membrane

2) MOPS buffer

a) pKa : 7.15 : It meets as pKa should be between 6-8

b) pKa / 0C = -0.013: the condition meets, the effect of temperature is minimal (<0.02 0C)

c) absorbs light < 230 nm : the condition meets as the absroption should be minimal from 240-700 nm and it is absrobing below the range

d) does not cross the bilipid layer: the condition meets as it has minimal permeability to biological membrane

So answer is

All of the above properties fullfill the Good buffer's criteria

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