Indicate the answer choice that best completes the statement or answers the ques
ID: 102462 • Letter: I
Question
Indicate the answer choice that best completes the statement or answers the question 1. Multi-cloning sites ("poly-linkers") in most commercially available plasmids offer all the followi over the earlier plasmids used in genetic recombination except: a. They can be used with a wide variety of restriction enzymes. b. They assure that only the desired DNA will be inserted into the plasmid. c. They enable easy control of the direction of insertion of the gene of interest d. They are usually adjacent to any necessary promoters for gene expression e. All of these are advantages Exhibit 13A This is a map of the pBR322 plasmid, numbers on the map refer to the base where the site is locate 29 Eoo21 4361 2963 amHI 375 651 Hien 3056 ORI Anli 2. Refer to Exhibit 13A. If a recombinant plasmid were obtained inserting DNA into the AIll site, the recombinant plasmid would lack which of the following properties? a. It can replicate autonomously b. It will be resistant to the antibiotic tetracycline. c. It will be resistant to the antibiotic ampicillin. d. It will possess at lest two promoter sites. e. All of these are true for the recombined plasmid. Page tExplanation / Answer
1) d)They are usually adjacent to nay necessary promoters for gene expression.
A polylinker is a short DNA sequence containing two or more different sites for cleavage by restriction enzymes
2) a) It can replicate autonomously
Removal of AfIII will result in mutations
3) d) all of these
In recombinant technology vector gene is inserted into the host cell, viral genome can be inserted into the bacterial genome using host.
4) c) Plasmid is a small circular DNA that is not part of bacterial chromosome.
5) c) Ligases
Ligase is like a molecular stitcher , is the enzyme that joins two single stranded DNA fragments
6) e) More than one of these colonies indicates that the plasmid contains recombinant DNA.
Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids grow into blue colonies.
7) When DNA evidence is used it is easier to prove that a person is guilty that ther are innocent.
DNA can be used to clear suspects and exonerate persons mistakenly accused or convicted of crimes.
A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA plasmids naturally exist in bacterial cells
8) Homologies
A couple of homologous chromosomes, or homolog, are a set of one maternal and one paternal chromosome that pair up with each other inside a cell during meiosis.
9) a) All of DNA of organism divided up and cloned in suitable vectors.
A DNA library is collectio0n of DNA fragments that have been cloned in suitable vectors.
10) c) sequencing of complementary DNA produced by reverse transcriptase.
RNAs are converted to cDNA that is used as the input to a next-generation sequencing
11) d) All of these
Epogen is trade name of erythropoietin produced by recombinant technology use to treat chronic kidney disease illegally used by athletes to boost performance.
12) True
Bacteria can splice introns. Therefore, no functional protein could be made
13)True
Glycosylation occurs in eukaryotes and widely in archaea, but very rarely in bacteria.
14)All of the above
Genetic engineering the direct manipulation of an organism's genome using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms
15) True
DNA microarrays to measure the expression level of large numbers of genes simultaneously or to genotype multiple regions of a genome.
16)
17) True
Ethidium bromide
Ethidium bromide is an intercalating agent Commonly used as a fluorescent tag
18) All of the above
Analytical ultracentrifugation, autoradiograph and X-Ray Crystallography are used to read protein and DNA structures separated by electrophoresis.
19) All of the above
Radioactivity, fluorescence, dyes which bind to DNA and luminescence are used to find the DNA binding in electrophoresis.
20) All of these make restriction enzymes useful
They are commercially available producing sticky and blunt ends