Prepare control group by adding _____ DPBS to the tube which contains 5 mu l of

Question

Prepare control group by adding _____ DPBS to the tube which contains 5 mu l of the "CON" Borrelia lysate at a concentration of 0.5 mg/ml. Your goal is to make a dilution at a final concentration of 5 mu g/ml of lysate in coating. 500 mu l 500 ml 495 mu l 495 ml Prepare experimental group by adding _____mu l of DPBS to the lysate tube. The tube contains 10 mu l of this experimental lysate at a concentration of 0.5 mg/ml and you want a final concentration of 5 mu g/ml to coat the plate. 9.9 mu l 990 mu 1 900 mu l 099 ml

Explanation / Answer

1. The answer is 495 ul

The difference between intitial and final concetration is 500- 5 = 495.

2. The answer is 990 ul

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