Column Chromatography. PLEASE HELP GC= Gas Chromatography Expt. 5a Quiz: Column
ID: 1033576 • Letter: C
Question
Column Chromatography. PLEASE HELP
GC= Gas Chromatography
Expt. 5a Quiz: Column Chromatography each component being analyzed by GC (toluene and cyclohexane) have a uve masses? (ie. Why can't you just use the peak areas to compare are using a thermal conductivity detector for our GC analysis. Why must GC chromatogram to 1. We specific correction factor to convert peak areas on the amounts of each compound?) (4 pts.) Draw the condenser and Label the proper places for H20 in and out on the condenser below. (4 pts.) 2. For each of the following techniques, identify the physical phase (solid, liquid, or gas) of the mobile and stationary phases used in that technique: (6 pts.) 3. Mobile Stationary TLC Column Chromatography GCExplanation / Answer
1)
It is important to correlate by a correlation factor of each peak in GC to quantify the amount and not directly use the area of the peaks. As we do the GC runs, the amount of sample injected varies with each sample as control over the exact volume is difficult. These thus lead to different areas for the compound in the GC.
Therefore, for better reproducibility we use a correlation factor that works on the elimination variability principle. All the variables that affects the area or response factor of the compound in the GC are taken care of by using a correlation factor.