Mixtures of amino acids can be analyzed by separating the mixture into its compo

Question

Mixtures of amino acids can be analyzed by separating the mixture into its components through ion- exchange chromatography. Amino acids placed on a cation-exchange resin containing sulfonate (-SO3) groups flow down the column at different rates because of two factors that influence their movement: (1) ionic attraction between the sulfonate groups on the column and positively charged functional groups on the amino acids, and (2) hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin. For each pair of amino acids listed, determine the order of elution from a cation exchanger in pH 7.0 buffer. Amino Acid Pair | utes First Elutes Second Glu and Arg Lys and Met Gly and Asp Ala and Leu Phe and Ser Gly AlaLeu SerPhe Arg Me Lys Asp Glu

Explanation / Answer

Mixtures of amino acids can be analyzed by separating the mixture into its compo

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