I\'m pretty sure it\'s \"I have desturbed tertiar structure by disrupting disulf

Question

I'm pretty sure it's "I have desturbed tertiar structure by disrupting disulfide bridges." But i'm not for sure

QUESTION 5 I have purified Delta and have an assay to determine if it binds Notch. I know after my purification steps Delta is in its native shape and binds Notch as my binding assay gives a positive response. In a separate assay, I add beta mercaptoethanol to my Delita before binding to Notch. I no longer get a positive response from my binding assay. What is likely to have happened? OI have unfolded and disrupted all secondary and tertiary protein structure. I have desturbed tertiary structure by disrupting disulfide bridges. OI am breaking peptide bonds cutting the protein into pieces. OI have interfered with hydrogen bonding in the backbone and disrupted secondary structure and thus tertiary but left covalent bonds intact. I haven't done any of these things.

Explanation / Answer

Reducing agents such as beta-mercaptoethanol disrupt sulfide bridges present in the protein structure thereby disturbing the secondary and tertiary structure of the proteins making them denatured and returning back to their native state. therefore, the correct option is I have unfolded and disrupted all secondary and tertiary protein structure.

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