If you want to get 10%wt protein membrane what volume of 2%wt of beta lactoglobu
ID: 131971 • Letter: I
Question
If you want to get 10%wt protein membrane what volume of 2%wt of beta lactoglobulin and how much activated carbon would you use. Please show your calculation and explain any assumption made.
Preparation of adsorber membranes. Hybrid composite adsorber membranes were prepared to adsorb the toxic heavy metal and radioactive pollutants. Typically, to yielda 2 wt% protein hybrid membrane, 5 ml of a 10 wt% dispersion of activated carbon was mixed with 0.5 ml of a 2 wt% ß-lactoglobulin (pH 2) protein fibril solution. A 1 ml volume of this solution was vacuum-filtered using 0.22 ?m cellulose filters (diameter, 25 mm). 7.4 wt% protein hybrid membranes were prepared by filtering 1 ml solution containing 5.7 mg ß-lactoglobulin fibrils and 71.5 mg activated carbon dispersion. 29 wt% protein hybrid membranes were prepared in the same way by vacuum filtering 1 ml solution containing 20.4 mg amyloid fibrils and 50 mg activated carbon dispersion. The extreme adhesiveness and stiffness of the protein fibrils enabled the homogeneous assembly of the hybrid amyloid fibrils-activated carbon composite adsorption membranes. These adsorption membranes were then used as is to adsorb heavy metal ion pollutants from environmentally polluted water and used for high-temperature reduction in the recovery of the heavy metals.Explanation / Answer
Ans.
Suppose x = mg of beta lactoglobulin
y = mg of activated carbon
As, % wt = wt of protein (i.e., beta lactoglobulin) / total wt (i.e., wt of protein + wt of activated carbon)
10 = (x / x + y) * 100
0.1 = x / x + y
0.1 x + 0.1 y = x
0.1 y = 0.9 x
x / y = 0.1 / 0.9.
so, 10 mg of beta lactoglobulin and 90 mg of activated carbon would be used to get 10 % wt protein membrane.