Design Layout References Mailings Revew View Prob. Set 5 Compatibility Mode]Q Se
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Design Layout References Mailings Revew View Prob. Set 5 Compatibility Mode]Q Search in Document Medgar Evers College Bio 302-Genetics- Problem Set 5 Prof. Small 11.25.13 1) a) Suppose you label dividing cells with 32P. In which DNA molecules will the radioactivity be incorporated if you were to look during metaphase? b) Suppose you are examining the above cells after replication. In which DNA molecules will the radioactivity be present? 2) In one picture, draw a schematic indicating how DNA is synthesized bi-directionally. The schematic should indicate which are the lagging strands, which are the leading strands, where the RNA primers are, and the 3' and 5 orientation of the strands. Indicate the Okazaki fragments in the figure. b) Why does the lagging strand have Okazaki fragments while the leading strand does not? 3) Suppose you had a bacterial mutant that was defective for topoisomerase, one that was defective for primase, and one that was defective for DNA polymerase. Your student mis- labelled the three cultures. How could you differentiate among the three mutants? 4) Describe two ways in which prokaryotic and eukaryotic replication differs. English (US) 169 WordsLExplanation / Answer
Answer (4) :-
Difference between prokaryotic and eukaryotic replication differs are :- In prokaryotic cells, there is only one point of origin, replication occurs in two opposing directions at the same time, and takes place in the cell cytoplasm.
Where as in eukaryotic cells on the other hand, have multiple points of origin, and use unidirectional replication within the nucleus of the cell. So these are the two basic difference between prokaryotic and eukaryotic replication .