Part IV: Extraction Protocols (4.0 Pts) You have been asked to investigate brini
ID: 132127 • Letter: P
Question
Part IV: Extraction Protocols (4.0 Pts) You have been asked to investigate brining a molecular test into your laboratory quantity of your extract 1. What factors should you consider when choosing an extraction protocols for nucleic acid extraction and ways to evaluate the quality and method? List five factors and explain why it would be an important consideration for your loborato 2. You know that the tests your lab will be performing will need isolated DNA. Which method of isolation will be the fastest and what draw backs might it have? How is it able to achieve fast resuits? What suggestions do you have for methods to assess the quality of your isolated DNA? How can you measure for purity? 3. What hazardous materials should you inform your QA officer that could potentially be brought into the laboratory if molecular testing is brought on as a testing methodology? 4. Part V-Gel Running Experiments (4.0 Pts) Calculate the DNA Quality given the following conditions and state if the extracted DNA is Acceptable or Unacceptable for further testing Sample 51173 50933 50808 OD2so Ratio Continue testing? OD260 178 452 323 0.158 198 189 When unsuitable the extraction procedure needs to be repeatedExplanation / Answer
1) Nucleic acid extraction involves three steps: breaking open the cells, removing other molecules such as amino acids and fatty acids and finally transferring the nucleic acids to buffer solution which will preserve them. The nucleic acids can be extracted either in solution based methods or solid phase based methods. The factors that are to be considered in in nucleic acid extraction are listed below,
Each of these help in extraction of specific nucleic acid sequences from others.
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