Can someone explain #2 and #3 for me? 1. How do you know which one is the ORF (w

Question

Can someone explain #2 and #3 for me?

1. How do you know which one is the ORF (what do you look for to find an ORF) and is wherever the ORF is will that be the coding strand as well?

2. How do you know which one is able to form a stable hairpin? What do you look for?

I really appreciate the help. Thank you. Will thumbs up if I can understand a good explanation :)

**My question is pretty clear. I just need someone to explain to me these concepts. Thanks for coming in clutch Chegg.

2. Genetie code. There is a single open-reading frame (ORF) in the DNA molecule shown below Recall that an ORF begins with a start codon and ends with a stop codon A) Find the ORF in one of the DNA strands. Draw a box around the ORF in its coding strand 5-TCTATCTCTCCTTGGCCATGTCGAATCCT-3 B) Translate the ORF into a polypeptide. (In the space provided below, write the protein's sequence. Use the amino acid residues N-terminus)MAKER (C-terminus) klet C) What is the net charge on this polypeptide at pH 74? net charge Which of the following RNA sequences can form a stable intramolecular hairpin? (Circle one letter A-E). Driw this RNA hairpin structure inside the box below Ga-C

Explanation / Answer

Ans 2..a...open readung frame start with ATG ( methionine)in mostly of the species and it end with a stop codon (TAA,TAG,TGA)

The selected sequence has ATG in 3' position and TAG in 5' position.

B..after transcription

ATG...code for methionin

GCC...Code for alanine

AAG...code for.lysin which is represented by K

GAG...code for glutamic acid which is repe3sented by E

AGA ...code for Arginine which is represented by R

Ans 3..menthioine and alanine has no charge

Lysin is positive

Glutamic acid is negative

Arginine is positive

So net charge is 1+

Ans 3...For hair pin like structure base pair must be compliment to each other

If u observe closely only option C has well no. Of complimentary base pair and the two opposite ends 5 and 3 which can form bond togetheer

Like 5 U is compliment with 3 A

5 G is compliment with 3 G

And so on

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