Incorrect Question 8 0/ 1 pts We want to identify whether the Apple protein asso
ID: 140350 • Letter: I
Question
Incorrect Question 8 0/ 1 pts We want to identify whether the Apple protein associates with any other factors in the cell. If the Apple protein is a human protein, can we still utilize the yeast 2 hybid system? Explain. Yes, because human and yeast genes share homology Yes, as long as we use a human specific transcription factor for both fusion proteins. Yes, as long as we attach the human proteins to the yeast transcription factors in both fusion proteins. No, because human proteins cannot be expressed in yeast. No, because human proteins cannot be properly folded in yeast No, because selection markers used for yeast aren't applicable to human proteinsExplanation / Answer
Yeast hybrid screnning (one hybrid, two hybrid, three hybrid system) is an molecular technique in molecular biology for DNA-protein, protein-protein intractions. Intraction between two domains of the proetin are known an yeast-two hyrid for testing intraction between two proteins. Expression of protein from downstrem reporter gene by binding of transcription factor with upstream activating sequence when two domian of protein called DNA binding domian and activating domain binds with each other with specific protein sequences will leads activation of transcription.
For the identification of apple protein yeast two hybrid system can be used because the protein domain will be varied accordingly to study protein-protein intraction and will be kept constant. However, the activation domain must have potential to activate trasnscription of reporter gene by using yeast cell transcription machinnery. Yes, yeast two hybrid system still be used to study intraction for which specific regions of transcription factors (of human) are used for binding of activation domain and DNA binding domian. A successful intraction between the fused protein directlys links up with the change in the phenotype of yeast cell.