What is a primary difference between PCR and traditional cloning procedures such
ID: 14051 • Letter: W
Question
What is a primary difference between PCR and traditional cloning procedures such as those used to clone the human growth hormone gene?A. PCR is more time-consuming, but the purity of the obtained DNA clone is much higher than in traditional cloning.
B. PCR uses plasmid vectors, whereas traditional cloning uses bacteria.
C. PCR eliminates the need for restriction enzymes, vectors, and cells.
D. PCR and traditional cloning make use of different types of vectors.
E. PCR and traditional cloning make use of different types of bacteria.
Explanation / Answer
Answer:
[C]
In PCR, you only unwind the helical twist of DNA and then amplify specific regions of it through enzymes. No need for the other stuff.