The most important and basic assumption of enzyme assays is: Select one: a. Reac
ID: 140563 • Letter: T
Question
The most important and basic assumption of enzyme assays is: Select one: a. Reactions cannot occur below the temperature optimum O b. Reaction rates directly relate to the amount of enzyme and substrate present c. Reactions cannot occur outside of the optimal pH O d. Reaction rates are directly related to the regulation of salt concentration The catalytic activity of an enzyme is least likely to be influenced by Select one: Oa. excess substrate b. chemical environment c. product d. three-dimensional structure Which of the following is the OPTIMUM pH for a particular enzyme? (Choose ALL that are possible!) Select one or more: a. above 7 (basic) ob. 7 (neutral) c. below 7 (acidic)Explanation / Answer
The correct option is option B) Reaction rate directly related to the amount of enzyme and substrate present.
Now the reaction occurs in the maximum velocity when the pH and temperature is optimal. But it also takes place below and above the optimal conditions to certain range.... Hence option A and C are ruled out.
Secondly the salt concentration doeendohave any rope in enzyme assay. Hence it's also wrong.
2) option A) excess substrate.
The enzyme kinetics is dependent directly on the amount of substrate taken. But as the substrate concentration increases, the reaction velocity reach asymptote and the substrate concentration doesn't have any further ripenin enzyme kinetics as all the ezyme molecules are saturated. Hence it's least likely to influence catalytic activity.
3) all the three optons are correct.
Enzymes like pepsin(1-2 pH) and acid phosphatase (4-5)
Enzyme alkaline phosphatase ( 10-11)
Fungi and plant enzyme (4-5)
The different enzyme has iferent pH requirement. Hence all the options are correct.