Submit Answer Answer Submitted: Your final submission will bee VI graded after t
ID: 140876 • Letter: S
Question
Submit Answer Answer Submitted: Your final submission will bee VI graded after the due date. Tries The two vesicle fractions are either untreated (-) or are incubated with the protease rypsin (+). The membrane vesicles are then washed, the membranes solubilized and he proteins denatured and analyzed by SDS gel electrophoresis and Western blotting using polyclonal antibodies against glycophorin A, an erythrocyte plasma membrane protein. The following data are obtained: ROV IOV ROV IOV Trypsin: -+ What can be deduced from these data about the structure of glycophorin A?Explanation / Answer
In both the cases, Glycophorin A is only partially digested by trypsin.
ROV will have the most portions of glycophorin outside, due to the SDS gel protein band. ROV+(small protein size) has the lowest band, which implies that only the large part of the protein was digested by trypsin. IOV+ (big protein size) has a higher band implying that only a small portion of the protein was digested by trypsin. As for Glycophorin A, its major portion is inside the erythrocyte and its minor portion is on the outside of the erythrocyte.