I just need to rewrite the abstract in your own words (professionally) Title : C
ID: 142334 • Letter: I
Question
I just need to rewrite the abstract in your own words (professionally)
Title : Caloric dose-responsive genes in blood cells differentiate the metabolic status of
obese men
Abstract :
We have investigated the postprandial transcriptional response of blood cells to increasing caloric doses of a meal challenge to test whether the dynamic
response of the human organism to the ingestion of food is dependent on metabolic health.
The randomized crossover study included seven normal weight and seven obese men consuming three doses (500/1000/1500 kcal) of a high-fat meal. The
blood cell transcriptome was measured before and 2, 4, and 6 h after meal ingestion (168 samples). We applied univariate and multivariate statistics to
investigate differentially expressed genes in both study groups.
We identified 624 probe sets that were up- or down-regulated after the caloric challenge in a dose-dependent manner. These transcripts were most
responsive to the 1500 kcal challenge in the obese group and were associated with postprandial insulin and oxidative phosphorylation. Furthermore, the data
revealed a separation of the obese group into individuals whose response was close to the normal weight group and individuals with a transcriptional response
indicative of a loss of metabolic flexibility.
The molecular signature provided by the postprandial transcriptomic response of blood cells to increasing caloric doses of a high-fat meal challenge may
represent a sensitive way to evaluate the qualitative impact of food on human health.
Explanation / Answer
Here, we have to investigate that whether the response of human body after the ingestion of food is dependent on his metabolic health i. e. well-functioning of metabolism in the body or not. To study that, we have measured here the changes in the transcriptional response (changes in mRNA components) of the blood cells at the period after having the meal, by subjecting the person to increasing caloric doses of a meal challenge. In this study, we included a random sample of seven normal weight and seven obese men and let them consume three doses of a high-fat meal; first dose being of 500 kcal, the second dose is of 1000 kcal and the third dose is of 1500 kcal. We then measured the total RNA content i.e. Transcriptome of the blood cells before and 2, 4, and 6 h after meal ingestion (now having a total of 168 samples) to see how genes got expressed with response to changes in the calorie content of the meals. To investigate differentially expressed genes in both ‘normal weight’ and ‘obese’ study groups an univariate and multivariate statistical analysis were carried out. We identified the specific genes with the help of their specific complementary probe and found that a total of 624 probe sets show up- or down-regulation after the caloric challenge in a dose-dependent manner. High number of transcripts of certain genes is found in the obese group having 1500 kCal meals, that means expression of those genes are upregulated. The obese group having 1500 kcal meals also showed a change in the level of blood insulin and also a change in oxidative phosphorylation (ATP formation) pattern slightly after the meals. Some data revealed, a fraction of obese people whose body response was close to the normal weight group despite having high calorie meal, also they showed less variation in their transcriptional response compared to normal weight persons, which is indicative of the fact that these individuals have less metabolic flexibility. Thus, by the changes that occurred at the molecular level (can be called as a molecular signature of the cells in a particular ‘treatment’ condition) in the transcriptomic profile of blood cells to increasing caloric doses of a high-fat meal challenge may represent an indicative way to evaluate the impact of the quality of food on human health.