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Following are events that occur enabling the synthesis of the lagging strand of

ID: 14395 • Letter: F

Question

Following are events that occur enabling the synthesis of the lagging strand of DNA during replication of the Escherichia coli chromosome. DNA ligase seals the nicks between adjacent fragments of DNA. Primase synthesizes short RNA primers. The RNA primers arc removed. Okazaki fragments are formed. Which one of the following correctly orders these events? 1, 2, 3, 4 2, 3, 4,1 2, 1, 3, 4 2, 4, 3, 1 In order to allow the synthesis of Okazaki fragments, the replisome must move backwards. the template strand with short RNA primers bound must loop back around through the replisome. there must be two different DNA polymerase enzymes at each replication fork. all of the above The following image shows a gene construct found in prokaryotes. The genes trpE, trpD, trpC, trpB and trpA encode for products needed to synthesize the amino acid tryptophane. All five of these genes are under control of the same promoter All of the following are true EXCEPT This is an operon The mRNA formed after the transcription of this construct will be polycistronic. The mRNA generated upon transcription of this construct would have the same sequence as the 3' to 5' strand except that Us would replace Ts. The S' to 3' strand is the coding strand. The TrpE gene is upstream of the TrpB gene.

Explanation / Answer

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The events should be e) 2, 4, 3, 1

c. is correct about the Okazaki fragments. Definitely - it's a complicated affair at the molecular level..

c. is incorrect
Because the mRNA would have the same sequence as the 5' to 3' strand, not 3' to 5' as stated.