All of the cells in my cell culture are floating in the media rather than sticki
ID: 145454 • Letter: A
Question
All of the cells in my cell culture are floating in the media rather than sticking to the bottom of the plastic dish. Fill in the blanks below that outline the steps of a very simple experiment to test if cells can actually stick to the dish or if they need some biological material to anchor them.
4. All of the cells in my cell culture are floating in the media rather than sticking to the bottom of the plastic dish. Fill in the blanks below that outline the steps of a very simple experiment to test if cells can actually stick to the dish or if they need some biological material to anchor them. Hypothesis Background information: Method*: Predicted result: Follow-up experiment based on results "what is a good candidate molecule to use to anchor the cells to the plate? (hint: look at ECM components for cells)Explanation / Answer
Hypothesis: To test whether ECM components can help in attaching the cells to the tissue culture plates.
Background information: It has been found by many researchers that treating the cell culture plates with extracellular matrix protein such as collagen Type I, fibronectin or vitronectin help the cells to attach via integrins and other cell surface receptors.
Method: Millipore now offers precoated multiwell plates in 6- and 24-well formats. Coatings include collagen, fibronectin, and poly-D-lysine. Precoated products offer a great advantage to researchers. There is no lengthy coating process, plates are coated by a consistent process and they are always available when needed.
Method:
Predicted result: After overnight incubation of cells on coated petriplates, next day you can observe the attachmnet of cells under the microscope. If there is any error in the coating of the collagen in the flask, then the cells might not adhered to the petri plates.
Follow-up experiment based on the result: The cells adhered to the petri plates can be treated with MTT dye or trypan blue to see the viability of cells on collagen treated plates.