The following statements describe SDS-PAGE, a method used to separate proteins.
ID: 145758 • Letter: T
Question
The following statements describe SDS-PAGE, a method used to separate proteins. Select the true statements. Assume that SDS-PAGE is performed under reducing conditions. Smaller proteins migrate faster through the polyacrylamide gel. Proteins are separated in a polyacrylamide gel matrix. A protein binds roughly 1.4 times its mass of SDS resulting in a large overall negative charge. Proteins are visualized using a dye that binds to the gel matrix, but not to proteins. Protein-SDS complexes migrate toward the negative electrode. Sodium dodecyl sulfate binds proteins, resulting in protein-SDS complexes that are similar in size.Explanation / Answer
I believe the correct choices from the above choices are:
A) Smaller proteins migrate faster through the polyacrulamide gel.
B) Proteins are seperated in the polyacrylamide gel matrix.
SDS page has somewhat similar mechanism of determining the size of the protein like a normal gel electrophoresis but here, the negative charge is provided by the SDS while DNA has its own charge to facilitate movement in one direction.
Feel free to leave a comment down below for any further query. Good rating would be appretiated if you find my answer helpful. Thank you.