In these experiments characterising pyruvate kinase you will often measure the p
ID: 146479 • Letter: I
Question
In these experiments characterising pyruvate kinase you will often measure the protein concentration of a solution using the method of Bradford. Does this measure the total protein concentration, or only the concentration of pyruvate kinase? Select one: a. Total protein concentration b. Pyruvate kinase concentration The enzyme glyceraldehyde 3-phosphate dehydrogenase catalyses the sixth step of glycolysis, the breakdown of glucose in eukaryotic cells. This enzyme uses glyceraldehyde 3-phosphate and NAD+ as substrates, producing the products NADH and glycerate 1,3-bisphosphate. In light of the assay for pyruvate kinase activity that you will perform, could this enzyme be assayed directly, or would it require the use of a coupled assay? Select one: a. Would require a coupled assay b. Can be directly assayed Using the coupled assay for pyruvate kinase activity, Christopher measured the total change in concentration of NADH for an assay to be 200 uM. How much PEP was consumed by pyruvate kinase (in to 3 st)? Answer:Explanation / Answer
1) Bradford's assay is a technique used to measure the total protein concentration in a solution. This method uses spectroscopic analysis and depends on the amino acid composition. Therefore, the answer for this question is option a.