Indicate whether the following descriptions apply to nutrient, defined, selectiv
ID: 146730 • Letter: I
Question
Indicate whether the following descriptions apply to nutrient, defined, selective, differential, or enrichment media. If the statement applies to more than one, indicate which ones.
Answers may be used more than once.
____________________ if you want to take an environmental sample and grow a wide variety of
bacteria, you would use this medium
____________________ blood agar is an example, because you can see whether or not microbes produce substances that lyse red blood cells
____________________ you might make this medium with a specially-formulated salt mixture, some vitamins, and a refined protein source like peptone
____________________ the lauryl tryptose broth that we used for the presumptive coliform test is this type of medium- we used it to increase the chance of actually seeing the coliforms
B. Indicate whether the following procedures are best performed with liquid or solid media. If the statement applies to both, say “both.”
_______________________ you can count colonies of microbes grown with this medium
_______________________ you can grow facultative anaerobes with this medium
_______________________ you can use a spectrophotometer to measure growth in this medium
_______________________ you can prepare this medium by autoclaving it prior to use
Explanation / Answer
Please find the answers below:
Answer 1: Nutrient media (it is a general purpose media which allows basic growth of nearly all type of microbes in a culture)
Answer 2: Differential media (a differentail media, such as blood-agar allows differentiation between some strains such as those which can lyse red blood cells against the ones which cannot lyse them)
Answer 3: Defined media (a defined emdia is the one which is enriched with some special nutrients which allow growth of some speific bacteria)
Answer 4: Selective media (this type of media selectively allows grows of some microbes but not others)