Microbiologists have learned how to isolate, cultivate, and characterize many di

Question

Microbiologists have learned how to isolate, cultivate, and characterize many different groups of bacteria Although there is no universal medium, microbiologist use what they know about specific groups to derive media formulations that fit their particular scientific goal. Knowledge of how media components are used to facilitate, or even hinder growth is a useful tool for studying microbes. Using the recipes on the next page fill out the table. Medium Nutrient Agar AIA APT+azide MacConkeys What components are these organisms using for an carbon source? What components are these organisms using for a nitrogen source? Is this medium considered selective If it is selective, what ingredient(s) is/are the selective agent? Is this a differential medium? If it is differential, what component (s) is/are helping differentiate microbes? Which groups may be cultivated on this medium? What would be a good source of inoculum for the group in the above row?

Explanation / Answer

1 Nutrient Agar

A. Peptone, beef extract

B. Peptone and beef extract

C. No

D. Nil

E. No

F. General media to grow different groups of bacteria

G. Beef extract and peptone

2 Ans Actinomycete Isolation Agar

A. Soluble starch

B. sodium caseinate

C. Yes

D. sodium caseinate, L-Asparagine, Sodium propionate, Dipotassium phosphate

E. No

F. Nil

G. Actinomycetes

H. Sodium caseinate

3 Ans. APT Agar

A. casein enzymic hydrolysate
B. casein enzymic hydrolysate

C. Yes

D. casein enzymic hydrolysate,

E. No

F. Nil

G.Lactobacillus

H. casein enzymic hydrolysate,

4. MACCONKEY AGAR

A. Lactose

B. Peptone and proteose peptone

C.yes

D. Bile salts and crystal violet for gram negative enterics

E. Yes

F. Lactose provides a source of fermentable carbohydrate, allowing for differentiation.

G. Gram negative enteric, Ecoli, salmonella

H. Peptones and lactose

Related Questions

Navigate

• Browse (All)
• Browse M
• Subjects

---