Il. Use the DNA sequence below, which encodes a eukaryotic gene to answer the fo
ID: 149785 • Letter: I
Question
Il. Use the DNA sequence below, which encodes a eukaryotic gene to answer the following questions. 21 71 121 31 41 GGCGTATAAA GCGACGACTG TAGACTGATG AGCCTATCCA TGGACGCGCC 51 ATGGCCCTGT AAGCGGTGCG ATGCAATAAA ACGCGTATCA GTCATTCAGC 101 GTAGTCTGAT GCCAGTCGAC TGC 61 81 91 a) Write down the corresponding sequences, find them in the sequence above and label them TATA box sequence Polyadenylation signal in corresponding mRNA Kozak's sequence in corresponding mRNA Start (initiation) codon in corresponding mRNA Stop (termination) codon in corresponding mRNA (label as TATA) (label as Poly-A) (label as Kozak) (label as start) (label as stop) b) Whatregion of the mRNA contains the open reading frame that will be translated into protein? 41-101 27-35 51-59 c) Where is the 3'UTR? Circle one 59-101 51-123 63-101 71-81Explanation / Answer
a) TATA box-Sequence 1 contains the TATA box which is DNA Sequence found in the promoter region of genes in eukaryotes. It is the general binding site of transcription factors and is involved in process of transcription by RNA polymerase. TATA box is followed by multiple repeated adenine sequence up to 25 base pairs.
Polyadenylation Sequence: Sequence 91 consist of poly A tail when the DNA Sequence is transcribed to RNA poly A tail produced which is addition of multiple adenine base pairs to growing RNA chain.
CAG UAA_ GUC G
Kozak Sequence: Sequence 61 indicates the kozak Sequence of mRNA which has the codons of GCC and start point in translation.
UUC GCC ACG C
Start codon: sequence 1 and 71 have the start codon for eukaryotic mRNA indicated in mRNA as AUU
CCG CAU AUU U
UAC GUU AUU U
Stop Codon: Sequence 11,21 and 81 have stops codon in mRNA with the codon as UGA,UAG
CGC UGC UGA_ C
AUC UGA_ CUA C
UGC GCA UAG_ U
b) Stretch 41-101
This stretch contains the both start AUU and stop codons UAA hence this stretch can be considered as open reading frame
c) 51-123
Sequence 51-123 contains the 3’-UTR containing the regions of poly A rail and stop codons.