16. The following is a segment of DNA containing a palindrome. CTACGAATTCGTCT GA

Question

16. The following is a segment of DNA containing a palindrome. CTACGAATTCGTCT GATGCTTAAGCAGA A restriction enzyme that recognizes this sequence produces breaks four nucleotides apart from the axis of symmetry in each strand. The break leaves a 5' overhang. Show the fragments that can be generated with the complementary single-stranded projections. a. b. A restriction enzyme that recognized this produces breaks two nucleotides apart from the axis of symmetry in each strand. The break leaves a 3' overhang. Show the fragments that can be generated with the complementary single-stranded projections

Explanation / Answer

a) 5' - CTACGAAAAGCAGA -3' a restriction enzyme that recognized this sequence produces breaks four nucleotides apart from the axis of symmetry in each strand. The break leaves a 5' overhang.

b) 3' - GATGCTTTTCGTCT -5'. A restriction enzyme that recognized thus produces breaks two nucleotides apart from the axis of symmetry in each strand. The break leaves 3' overhang.

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