To examine the directionality of a kinesin motor that you have purified, you att
ID: 15256 • Letter: T
Question
To examine the directionality of a kinesin motor that you have purified, you attach the motor to a microscope slide. As your motility substrate, you add red fluorescent taxol-stabilized microtubules that have been used to nucleate assembly of green fluorescent tubulins. You add these red/green microtubules to the slide in the presence of ATP. What do you observe?1. The microtubules move with red fluorescence at the front end of the microtubules.
2. The microtubules move with green fluorescence at the front end of the microtubules.
3. The microtubules move, and the green and red together produce yellow.
4. No motility since microtubules require GTP.
5. None of the above.
Explanation / Answer
To examine the directionality of a kinesin motor that you have purified, you attach the motor to a microscope slide. As your motility substrate, you add red fluorescent taxol-stabilized microtubules that have been used to nucleate assembly of green fluorescent tubulins. You add these red/green microtubules to the slide in the presence of ATP. What do you observe?
1. The microtubules move with red fluorescence at the front end of the microtubules.
2. The microtubules move with green fluorescence at the front end of the microtubules.
3. The microtubules move, and the green and red together produce yellow.
4. No motility since microtubules require GTP.
5. None of the above