Consider cargo proteins that are destined for secretion to the extracellular spa
ID: 163339 • Letter: C
Question
Consider cargo proteins that are destined for secretion to the extracellular space. For each of the following pairs of mutations or conditions, predict where the cargo will end up (ex. in the cytoplasm, a specific organelle, transport vesicles, multiple places, extracellular space) for: (i) & (ii) Each individual condition; & (iii) The combination of both.
(i) Normal Sar1 is mutated so it binds tightly to GDP and cannot exchange it for GTP.(This leads to Sar1*-GDP being the only form of the protein in the cell.)
(ii) A transmembrane domain is added to the cargo protein.
(iii) Both
Explanation / Answer
The combination of both mutated so to bind withGDP and stop the exchange of GTP.
A transmembrane domain is added to the cargo protein