Assume that you were growing cells in culture and had determined the cell-cycle

Question

Assume that you were growing cells in culture and had determined the cell-cycle time to be 24 hours. You introduce 3H thymidine and prepare autoradiographs of metaphase chromosomes after 48 hours. You expect two classes of the chromosomes: one class that had completed one S phase in the label, and a second class that had completed a cellular division and an additional S phase in the label.

Complete the DNA (double-stranded) labeling pattern for chromosome that you would expect to find in metaphase chromosome having replicated once in label.

Please explain! :)

Explanation / Answer

ANS- After the completion of one replcation with the labelling of 3H Thymidine and autoradiography the metaphase chromosomes will consist of one old and and one new chromatids (labelled).

After going through the two replication the metaphase chromosomes will consist of-one chromatits-1 old amd one newother one two two new strands (both labelled) i.e. final final stage will be one mixed chromosomes and other strands are new.

new/ new strands

new/old strands

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