Match the techniques to solve the following specific problems: a. Determine if g
ID: 166225 • Letter: M
Question
Match the techniques to solve the following specific problems:
a. Determine if gene A is in an open chromatin (actively transcribed).
b. If gene B DNA is methylated.
c. Remove or reduce the function of gene A from an organism
d. If where nucleosomes are binding to the piece of DNA
e. The relationship between gene A and gene B in a pathway
f. If cells have decided to commit suicide due to unrepaired UV damage
Techniques:
(1) HpaII restriction digestion and running a gel
(2) Chromatin immunoprecipitation
(3) DNA footprint
(4) TUNEL assay
(5) Construct double mutants
(6) RNAi
Explanation / Answer
Match the techniques to solve the following specific problems:
a. Determine if gene A is in an open chromatin (actively transcribed). - DNA footprint
b. If gene B DNA is methylated. - HpaII restriction digestion and running a gel
c. Remove or reduce the function of gene A from an organism- RNAi
d. If where nucleosomes are binding to the piece of DNA - Chromatin immunoprecipitation
e. The relationship between gene A and gene B in a pathway - Construct double mutants
f. If cells have decided to commit suicide due to unrepaired UV damage - Tunnel Assay