Microfilament dynamics inside of cells are very different from that of test tube
ID: 166659 • Letter: M
Question
Microfilament dynamics inside of cells are very different from that of test tube reaction. It is mainly because of actin binding proteins. Select all that applies.
A. Arp 2/3 complex generates branched tree-like F-actin structures in the leading edge of migrating cells B. The concentration of actin inside of cells is far greater than critical concentration C. The activation of formins results in the formation of undisrupted long microfilament structure D. Arp 2/3 complex promote the polymerization of F-actin at the minus end E. Microfilament cross-linking protein filamin is most active in trailing edge of migrating cellsExplanation / Answer
A. Arp 2/3 complex forms tree like migarting structures while the newly migrating cell works, The Actin-relatated protein (Arp) thus helps the cell at the microfilament subunit point.
B. The actin filaments and other intermediate filaments are important and they overall organise cell's interior structure, the subunit pool of that subsequently having higher concentration than the critical concentration.
C. Formins are mostly Rho-GTPase effector proteins, they help regulate the actin and microtibule cytoskeleton.