Place the labels in the correct column Mature mRNA from a eukaryotic cell will n
ID: 168251 • Letter: P
Question
Place the labels in the correct column Mature mRNA from a eukaryotic cell will not be identical to the primary transcript pre-mRNA would be useful for an in vivo translation assay Eukaryotic pre-mRNA can be used for protein expression in prokaryotes RNA polymerases demonstrate the statement "form follows function" A eukaryotic promoter will be functional in a prokaryotic cell A prokaryotic promoter will be functional in eukaryotic cells Mature mRNA from a prokaryotic cell will be identical to the genomic sequence Reset True FalseExplanation / Answer
Ans. A. True:
I. Mature mRNA from a eukaryotic cell will not be identical to the primary transcript.
Because primary transcript has introns whereas mature mRNA has no introns. Moreover, mature mRNA has 5’-cap and 3’-poly A tail, both of which are not present in primary transcript.
II. RNA polymerase demonstrates the statement “form follows function”.
III. Mature mRNA from a prokaryotic cell will be identical to the genomic sequence. The statement is correct when seen with respect to the similarity of eukaryotic mRNA to its genome. Eukaryotic mature mRNA is not identical to (assuming complementarity is retained/ considered while comparison) its respective gene because introns are removed and even alternate splicing occurs- that altogether result an mRNA sequence different from the complementary form of the template strand.
Because there no modification of mRNA after transcription in prokaryotes, its sequence is exactly the same as the complementary template strand.
B. False:
I. Primary transcript cannot be translated because 5’-cap is not present in it. 5’cap is crucial for translation of mRNA as it facilitates binding to ribosome in correct orientation in mature mRNA.
II. Eukaryotic pre-mRNA (primary transcript) can be used for protein expression in prokaryotes. Pre-mRNA can’t be translated to give a functional protein because it consists of introns- the non-coding regions on pre-mRNA.
III. A eukaryotic promotor will be functional in a prokaryotic cell. Eukaryotic promotors are different from those of prokaryotes. Eukaryotic gene promotors can bind to eukaryotic transcription factors only. Since the bacteria lacks eukaryotic transcription factors, the promotor can’t associate with them. So, absence of eukaryotic transcription factors results NO transcription of the gene.
IV. A prokaryotic promotor will be functional in eukaryotic cells. Prokaryotic promotors are different from eukaryotic promotor. In absence of sigma-factor and prokaryotic RNA polymerase dependent of sigma-factor for initiating transcription, the prokaryotic promotor can’t be bound by eukaryotic RNA polymerases.
Note: Few points (A- II, and III) in the given options are not exact and precise in their meaning. Their validity may depend on the perspective they are explained. However, the options are placed in the most appropriate group with their respective explanation.