How is GFP attached to a protein of interest? A. The GFP DNA sequence is placed
ID: 171208 • Letter: H
Question
How is GFP attached to a protein of interest? A. The GFP DNA sequence is placed next to the protein's gene in the recombinant DNA so that they are eventually translated together. B. GFP and the protein of interest are separately purified using recombinant DNA technologies, then fused together using chemical cross-linkers. C. The cells containing the protein of interest are washed with GFP and it adheres to only the protein of interest. D. GFP is attached to a secondary antibody that binds a primary antibody attached to the protein of interest. E. They are purified together using TAP-tag based chromatography.Explanation / Answer
The correct answer is D , i.e. GFP is attached to secondary antibody that binds a primary antibody attached to the protein of interest .
because protein is to bind GFP to an antibody that will bind to the target protein. The direct method would bind the fluorescent antibody to the corresponding antigen on the target protein. It binds a non-fluorescent antibody on the target protein. Then the fluorescent antibody is introduced which will bind to the non-fluorescent antibody. Although it is possible to stain specific parts of cell directly, indirect methods are preferred due to high affinity.