An Hfr strain that is met^+ pro^+bio^+ his^+ was mated with an F- strain that wa

Question

An Hfr strain that is met^+ pro^+bio^+ his^+ was mated with an F- strain that was met^+ pro^+ bio^+ his^+ Mating was interrupted and the percentage of cells with each phenotype was determined by replica plating the cells on minimal media plates that lacked met, pro, bio or his. The data shown in Figure 1 were obtained. The distance between genes is measured by the amount of times in minutes it takes for the gene to be transferred during conjugation.A new gene can be placed in two possible position on the gene map based on the time in minutes it takes for the gene to be transferred relative to genes already on the map. In another experiment, the thrgene was found to be located 1 minute from the progene. In which locations(s) in the gene map could the thr gene be located? (Note that the distances depicted between the genes on the map are not to scale

Explanation / Answer

The correct options are C and D

since the distance between the His and Pro genes are a slight more than a minute so at the end of 12 minutes 15 % of cells have expressed His genes while 2 % of the cells have expressed pro gene. Also, the distance between Pro and Bio genes are more than 1 since it is only after 3 minutes do we see cells expressing Bio gene.

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