The design of the kinetics approach allowed quantitative determinations of the e
ID: 174384 • Letter: T
Question
The design of the kinetics approach allowed quantitative determinations of the effect of the pesticide as a potential inhibitor of tribbles proteins in the carnitine shuttle. Specifically, K_m and V_max values were compared across varying concentrations of pesticide. Data points from the Lineweaver-Burke plots were mathematically transformed, by taking the reciprocals of the points, to determine the K_m and V_max values. The data showed that the V_max of tribbles CAT I remained unchanged at 100 nmol/min/mg, whether the pesticide was present or not. Interestingly, the K_m values of the enzyme increased with increasing concentration of pesticide, from 33 mu M without pesticide to 200 mu M at the greatest pesticide concentration. The parameter values for CAT II and the transporter were unaffected by the pesticide. New Experiment Based on the above results, in vivo studies have now been approved, to determine the intermediate metabolic outcomes of inhibiting CAT I in tribbles when they strenuously exercise. Exercise Stress Test - Tribbles were fed com from the Iowa farm for three days. After an overnight fast, the tribbles were transferred to exercise wheels for 90 minutes of exercise. At the time points plotted in the charts below, blood samples were taken to determine levels of four metabolic intermediates: glucose, triacylglycerol (TAG), albumin-bound fatty acid, and ketone. Please respond to both questions using the single text box below. What additional evidence have you obtained from the new experiment? In other words, what were the results of the new experiment? Based on all of the information available in this case, how do you interpret the results and integrate them within the context of the original task? In other words, what is your conclusion about the biochemical mechanism that explains this case? A complete integration includes: Whether the new experimental design was appropriate Statement of the specific comparison being made in the new study Appropriate quantitative application At least two results from the new study Mention of all relevant results Explanation of the biochemical relationship between results Main conclusion; interpretation of the results within the context of the problemExplanation / Answer
1) From the nerw experiment it is evident that the utilization of blood glucose and fatty acids is increased and it can be known by their concentrations in the above given graph. Since, glucose and fatty acids are substrates, their utilization have been increased during strenuous exercise. The concentrations of plasma TAG (triacylglycerol) and ketones are found to be decreased. This is because, during exercise the metabolism is increased utilizing the intermediary products. Therefore, concentrations of the intermediates is varied.
2) The experimental design was appropriate. Role of the enzyme and utilization of substrates and intermediate products during metabolic process and effect of inhibition of enzyme CAT I was studied by this experiment.
The concentrations of various metabolic substrates and intermediates after feeding on pesticide treated corn and strenuous exercise for 90 minutes were measured.
The concentrations of glucose and fatty acid have been increased while the concentrations of TAG and ketones were decreased.
Inhibition of CAT I icreased the utilization of glucose and albumin linked fatty acids as substrates and the strenuous exercise of 90 minutes resulted in decreased concentrations of TAG and ketones.
Therefore, it can be concluded that the presence pesticide in the feed and inhibition of enzyme CAT I had shown little or no effect on metabolic activity during strenuous exercise of trills for 90 minutes.