Above is my data from a gel electrophoresis lab. Highlighted is the terminal vel
ID: 174531 • Letter: A
Question
Above is my data from a gel electrophoresis lab. Highlighted is the terminal velocity ratio of the proteins myoglobin/BSA, what formula gives the error propogation of this terminal velocity ratio?
Time Distance BSA N Time Distance MGR N Measured Velocity Ratio kDa Diff (sec) (mm) 11 (sec) (mm) 2.82899 slope Accepted Velocity Ratio kDa slope (mm/sec) (mm/sec) 3.647058824 0.007617 0.026994. 240 12.2 intercept intercept -0.10317506 (mm) 300 15.4 (mm) 360 14.9 12.99628 360 17.9 8.495499 485 16.2 485 205 600 17.9 0.976342 600 26 0.987454 726 19.2 726 29.9 s, 840 19.9 (mm) 840 32.6 (mm) 960 20.7 0.498205 960 35.5 1.421908 21.5 1086 38.5 Sgope 1086 1196 22.1 (mm/sec) 1196 40.8 (mm/sec) 23.0 0.000395 1328 43.8 0.000917 1328 Sntercept 46.5 Sintercept 23.7 1440 1440 1566 24.5 mm) 1566 49.2 (mm) 0.408979 0.878707Explanation / Answer
The terminal velocity ratio shows that distance migrated by myoglobin is more than the distance migrated by BSA. Velocity ratio is given (=3.64). Uncertainty in velocity ratio is also given (=0.103= given in G6).
Error propagation is under-root of sum of uncertainties in different values, present in row labelled ‘G’.
Q = [(G6) 2 +………..]1/2
**** Hope it helps...