A gourmet chef inoculated a delicious potato salad with 6 Staphylococcus aureus

Question

A gourmet chef inoculated a delicious potato salad with 6 Staphylococcus aureus cells. If S. aureushas a generation time of 60 minutes, how many cells would be in the culture after 10 hours. Use the dilution scheme shown below and answer the questions that follow. 1.0 mL Original Sample 0.1 mL 0.l mL 0.1 mL 1.0 mL 1.0 mL 9.0 mL 0.9 mL 9.9 mL 9.9 mL 9.0 mL 9.0 mL I II III IV V VI 0.1 mL 0.1 ml 48 colonies 50 colonies Calculate The dilution of each tube in the series. The approximate concentration of viable cells in the original culture. Suppose that the original sample had a concentration of 1.0 times 1010 cells/mL. Based on the latter concentration, how many cells are in tubes I, II, Ill, IV, V, and VI?

Explanation / Answer

1. Generation time of a bacteria refers to the time in which te bacterial cell get doubled.

So as initial cells are 6.

Generation time is 60 mins -1 hr

So after 10 hr.

1-2-4-8-16-32-64-128-256-512-1024 for 1 cell.

For 6 cells- 6×1024= 6144 cells.

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