Question
Please answer the whole thing, but there's ONE part in particular that's going to make my head explode. Part 1.C. Is she asking WHERE THE RIBOSOMES THAT PRODUCE RNA POLYMERASE THAT PRODUCE MRNA THAT PRODUCE CHAPERONE PROTEIN ARE LOCATED? What an asanine question.
Thanks!
1) Two point for correctly filling in the information at the top of every page. If you are not in a lab, write "none" where it asks for the lab section. If you don't know the lab section, day and time or TA will suffice 2) This exam is worth 100 points. The point value for each question is in parentheses. 3) You have 50 minutes to complete this exam. Ifyou get stuck on a question, move on to an easier question and return to the more difficult questions later. (Do the easy ones first!) Q1. The UPR pathway is important for sensing misfolded proteins in the ER- Your friend studies the UPR pathway in guinea pigs and finds that the Upr4 gene regulatory protein is phosphorylated on serine 152 when the UPR pathway is activated. When Upr4 is phosphorylated, it will go into the nucleus and bind to the promoter of the chaperone gene, leading to the production of chaperone mRNA. This pathway is diagrammed on the Figure below. This pathway becomes active when there are misfolded proteins within the ER. i For the following members of this A pathway, indicate the location of the misfolded ribosomes that translate each of these chaperone protein proteins. (2 points each, 8 points total) ER LUMEN properly folded a) Upr4. protein activated receptors activated kinase b) The "properly folded protein" near the orange arrow labeled "A") CYTOSOL c) The enzyme in the nucleus that synthesizes the chaperone mRNA chaperone gene NUCLEUS d) The receptor that binds to misfolded chaperone mRNA proteins ii) What is the name of the enzyme in the nucleus that synthesizes the chaperone mRNA? (2 points) iii) What is the name of the structure that the blue arrow labeled "B" is pointing to? (2 points) iv) What is the name of the cytoskeletal element that is just next to the inner membrane in the nucleus? (2 points) (Question 1 continues on next page)
Explanation / Answer
Q1 i): the location of ribosomes is also present free in cytosol and freely works as site for translation. The attachments for ribosomes are also Endoplasmic Reticulum and nuclear envelop.
Q1 ii): the enzyme is RNA polymerase II; this present inside the nucleus and makes RNA
Q1 iii): Nucleopore complex: these are gateway of nucleus, through the transportation of protein used.
Q1 iv): that is Endoplasmic Reticulum which is next to nucleus.