Microbiology : The trp operon of E. Coli is regulated by the Trp repressor prote

Question

Microbiology : The trp operon of E. Coli is regulated by the Trp repressor protein (encoded by the trpR gene) as well as a transcriptional attenuator (the Trp leader sequence). For each condition below, please write in the table whether the tryptophan biosynthetic enzymes would be produced: + indicates expression, - indicates no expression.
A) wild-type situation B) trpR is deleted, such that no Trp repressor is present in the cell C) the Trp codons in the leader sequence is changed to Ala codons, and alanine is abundant D) trpR is deleted, the Trp codons in the leader sequence is changed to Ala codons, and alanine levels are low
( table below) The trp operon of E. coli is regulated by the Trp repressor protein (encoded by the trpR gene) as well as a transcriptional attenuator leader sequence). For each condition below, write in the table whether the tryptophan biosynthetic enzymes would be produced: indicates expression indicates no expression. points) (A) wild-type situation (B) trpRis deleted, such that no Trp repressor is present in the cell (C) the Trp codons in the leader sequence is changed to Ala codons, and alanine is abundant (D) trpRis deleted, the Trp codons in the leader sequence is changed to Ala codons, and alanine levels are low high tryptophan levels low tryptophan levels Condition

Explanation / Answer

Answers:

Condition Low Tryptophan Level High Tryptophan Level A + - B + - C + - D + +

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