Hi I have lab and we\'ll clean cytochrome. I have some questions about the lab 1
ID: 175969 • Letter: H
Question
Hi I have lab and we'll clean cytochrome. I have some questions about the lab1. In what way cytochrome c binds to membranes? 2. In the lab we'll reduce cytochrome c's bilding ability to the membranes, why we'll do it ? 3. After the first spin where can I find cell debris, resolve proteins, precipitated proteins, salts and fata? In pellet or in supernatant ?
Please answer me quichly
Hi I have lab and we'll clean cytochrome. I have some questions about the lab
1. In what way cytochrome c binds to membranes? 2. In the lab we'll reduce cytochrome c's bilding ability to the membranes, why we'll do it ? 3. After the first spin where can I find cell debris, resolve proteins, precipitated proteins, salts and fata? In pellet or in supernatant ?
Please answer me quichly
Hi I have lab and we'll clean cytochrome. I have some questions about the lab
1. In what way cytochrome c binds to membranes? 2. In the lab we'll reduce cytochrome c's bilding ability to the membranes, why we'll do it ? 3. After the first spin where can I find cell debris, resolve proteins, precipitated proteins, salts and fata? In pellet or in supernatant ?
Please answer me quichly
I have lab and we'll clean cytochrome. I have some questions about the lab
1. In what way cytochrome c binds to membranes? 2. In the lab we'll reduce cytochrome c's bilding ability to the membranes, why we'll do it ? 3. After the first spin where can I find cell debris, resolve proteins, precipitated proteins, salts and fata? In pellet or in supernatant ?
Please answer me quichly
Explanation / Answer
Please find the answers:
Answer 1:Cytochrome c is a mitochondrial inner membrane bound protein with haeme moiety in its structure. This enzyme is physically bound to the inner side of mitochondrial membrane thus plays a crucial role in electron transport chain. The physical binding of cytochrome c to inner membrane is enhanced by binding of cytochrome c to cardiolipin.
Answer 2: Cytochrome c is involved not only in electron transport chain but also mediates many celluar functions such as apoptosis, ROS leakage, proton pump maintenance etc. Thus, in order to understand its structural features and patho-physiological impact of release of cytochrome c from mitochondria, a cellular preparation in which the cytochrome c has reduced binding ability with the inner membrane might have been produced.
Answer 3: After lysis of cells in a lysis solution and spinning at high speed, the supernatant will contain total protein fraction and the pellet will contain debris. This debris contains plasma/nuclear/mitochondrial membrane. Total protein can be precipitated from the suspension and the pellet can be re-processed for isolation of mitochondrial fraction. Ultra high-speed centrifugation will yield a pellet containing mitochondrial fractions.