There are several steps that are crucial for the efficient updtake of plasmid DN
ID: 177383 • Letter: T
Question
There are several steps that are crucial for the efficient updtake of plasmid DNA by bacterial cells and subsequent growth of the cells containing the plasmid. Place arrange each of these steps in sequential order. Remember, they are NOT necessarily listed in the order you would do them during the procedure.
A. Heat shock of bacteria at 42ºC for 90 seconds
B. Incubation of bacteria with plasmid on ice
C. Incubation of cells/DNA at 37ºC for 1 hour
D. Spread cells onto agar plates
What is the purpose of the heat shock step during the above procedure?
To facilitate the attachment of the plasmid DNA to the outer surface of the bacteria
To facilitate the entry of the plasmid DNA into the bacteria
To improve the efficiency of the enzyme digestion
Unknown
To facilitate the attachment of the plasmid DNA to the outer surface of the bacteria
To facilitate the entry of the plasmid DNA into the bacteria
To improve the efficiency of the enzyme digestion
Unknown
Explanation / Answer
To facilitate the entry of the plasmid DNA into the bacteria. The heat shock produces a pressure difference inside and outside the cell and it creates a hole in the cell and helps the entry of the plasid into the cell, and once the temparature becomes normal the hole will be sealed.