Certain mutations called amber in bacteria and viruses result in premature termi
ID: 179041 • Letter: C
Question
Certain mutations called amber in bacteria and viruses result in premature termination of polypeptide chains during translation. Many amber mutations have been detected at different points along the gene coding for a head protein in phage T4.
How might this system be further investigated to demonstrate and support the concept of colinearity?
Match the Bold phrases
5'
3'
longer
shorter
Mutations occurring near the ______ end of a gene will produce proteins with defects near the Nterminus. The lesions cause chain termination; therefore, the nearer the mutations are to the ______ end of the mRNA, the ______ will be the polypeptide product and thus the demonstration of the colinear relationship of genes and proteins.
Explanation / Answer
3' End. As the translation occurs in 5'-3' direction hence if the 3'is end gets mutated it will affect the N-terminal..
5' End
Shorter as the mutation result in the stopping of translation hence the stop codon are found due to which it results in formation of shorter length of protein which can also be truncated in nature and can be loss of function.