I) During gel electrophonesis, positively charged proteins will move to which (3
ID: 179519 • Letter: I
Question
I) During gel electrophonesis, positively charged proteins will move to which (3pts) wire? anode pants on fire cathode Copper Heat is used to prepare proteins before loading them on to sDs acrylamide gels. This is because high 3pts) heat will catalyze precipitation reactions so that the protein can be seen easier quicker the rate of gel polymerization inactivate proteases that could interfere with results denature the proteins 3) Bartonium exits a gel filtration column in 120 seconds while Bulldogium exits in 800 seconds. Which of the (3pts) following conditions describes the relationship between the two proteins? Bulldog um is larger than Bartonium Bartonium is larger than Bulldogium Bartonium is more hydrophobic than Bulldogium Bulldogium is less charged than BartoniumExplanation / Answer
1. Electrophoresis is a widely used analytical technique for the separation of biological molecules such as plasma proteins, lipoproteins, and immunoglobulins.
The movement of charged particles (ions) in an electrical field resulting in their migration towards the oppositely charged electrode is known as electrophoresis. Molecules with a net positive charge (cations) move towards the negative cathode while those with net negative charge (anions) migrate towards positive anode.
Hence, the correct answer is the cathode.
2. Heating is a process simply speeds up the process of denaturation by increasing molecular motion and mostly it is necessary for membrane samples. Heating to the boiling point can cause aggregation of proteins, defeating the purpose of SDS-PAGE. Inadequate heating can leave some proteins incompletely denatured. Once denatured, the samples can sit on a bench top at room temp until it is time to load them. If they are to be saved for another day, they should be frozen. If the samples are heated without first mixing with sample buffer, they will indeed be denatured, but not in the intended manner.
9. The assay is an analytical or investigative procedure in a laboratory for quantitatively measuring or qualitatively assessing the presence or amount or the functional activity of a target entity or the analyte.
Hence, the testing fo a single component of a larger mixture can be called as an assay.
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