If the length of the resulting real-time PCR products are identical use the melt
ID: 179688 • Letter: I
Question
If the length of the resulting real-time PCR products are identical use the melt curve data below to profit which one of the following peaks would correspond to the PCR product that has more G-C basepairs in it. The peak labeled A would have the greater G-C basepairing. The peak labeled B would have the greater G-C basepairing. The peak labeled C would have the greater C-C base pairing. The peaks labeled A and B would have the greater G-C basepairing. The peaks labeled C and B would both have the greatest G-C basepairing.Explanation / Answer
Answer:
1). E). 18:29, 12
The carbon that attcached to -COOH is called alpha-carbon. The carbons can be counted from the alpha-carbon, as c1, c2, c3, etc.
So total 18 carbons are present and double bonds are found between C9 & C10 and C12 & C13.
2).D). The peaks labeled A and B would have the greater G-C basepairing.