Membranes can be \'stripped\' of the antibodies and re-used. Based on what you k
ID: 179755 • Letter: M
Question
Membranes can be 'stripped' of the antibodies and re-used. Based on what you know about protein-protein interactions, what must occur for the antibodies to be stripped away? Identify a reagent that can be used in this stripping procedure. Why is the secondary antibody used? In other words, why not enzyme-link the primary antibody itself? What is an epitope? Is Western blotting (immunoblotting) a chromatographic technique? Explain your reasoning. Describe how to make a specific antibody such as one directed against RelA.Explanation / Answer
3) An epitope, also known as antigenic determinant, is the part of an antigen that is recognized by the immune system, specifically by antibodies, B cells, or T cells. For example, the epitope is the specific piece of the antigen to which an antibody binds. The part of an antibody that binds to the epitope is called a paratope.