Replication:: 6. What is the order of enzymatic events in E coli involved in the
ID: 179858 • Letter: R
Question
Replication::6. What is the order of enzymatic events in E coli involved in the synthesis of an Okazaki fragment and maturation to high molecular weight DNA ?
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7. What is the chemical difference between the groups joined by a DNA polymerase and a DNA ligase? ------------
8. Which enzymatic activity of E coli ploymarase I is responsible for removal of mismatched base pairs?
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9. Why is telomerase classified as a reverse transcriptase?
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10. Eucarytoic genomes are made up various frequency classes of DNA (highly repetitive, middle repetitive, unique) at differ in co lpy number. If you purified Okazaki fragments from a human liver cell and mixed them with denatured genome DNA, to which frequency class(es) of DNA would the Okazaki fragments hybridized?
Replication::
6. What is the order of enzymatic events in E coli involved in the synthesis of an Okazaki fragment and maturation to high molecular weight DNA ?
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7. What is the chemical difference between the groups joined by a DNA polymerase and a DNA ligase? ------------
8. Which enzymatic activity of E coli ploymarase I is responsible for removal of mismatched base pairs?
--------------
9. Why is telomerase classified as a reverse transcriptase?
-------------
10. Eucarytoic genomes are made up various frequency classes of DNA (highly repetitive, middle repetitive, unique) at differ in co lpy number. If you purified Okazaki fragments from a human liver cell and mixed them with denatured genome DNA, to which frequency class(es) of DNA would the Okazaki fragments hybridized?
6. What is the order of enzymatic events in E coli involved in the synthesis of an Okazaki fragment and maturation to high molecular weight DNA ?
---------
7. What is the chemical difference between the groups joined by a DNA polymerase and a DNA ligase? ------------
8. Which enzymatic activity of E coli ploymarase I is responsible for removal of mismatched base pairs?
--------------
9. Why is telomerase classified as a reverse transcriptase?
-------------
10. Eucarytoic genomes are made up various frequency classes of DNA (highly repetitive, middle repetitive, unique) at differ in co lpy number. If you purified Okazaki fragments from a human liver cell and mixed them with denatured genome DNA, to which frequency class(es) of DNA would the Okazaki fragments hybridized?
Explanation / Answer
6.it involves 3 major steps
Initiation
Elongation
Termination
7. dna ligase catalyses the joining of fragments of double stranded dna together. it can join blunt ends and sticky ends.
And dna polymerase catalyses the joining of free dna nucleotides to a chain of nucleotides with phosphodiester bonds (i.e forms the backbone of a single strand). it can only join the nucleotides if there is already a chain there for the free nucleotides to attach to...
8. Proofreading activity of dna polymerase identifies the errors and fix it.
9.Telomerase reverse transcriptase. ... To be specific, TERT is responsible for catalyzing the addition of nucleotides in a TTAGGG sequence to the ends of a chromosome's telomeres. This addition of repetitive DNA sequences prevents degradation of the chromosomal ends following multiple rounds of replication.