I need help with the final question that is below letter c Question 2 Below is t

Question

I need help with the final question that is below letter c Question 2 Below is the double-stranded DNA sequence of part of a hypothetical yeast genome, which happens to contain a very small gene. Transcription starts at the Transcription Start Site (TSS) after the promoter (shown in yellow), and proceeds in the direction of the arrow. Transcription stops at the end of the Transcription Terminator (shown in blue). TSS 5' AGCCATGCATTATCTAGATAGTAGGCTCTGAGAATTIATCTCACT-3 TITITI1111TISTTTTSTTTIT CTCGGTACGTAATAGATCTATCATCCGAGACTCTTAAATAGAGTGA-5 3'-G promoter terminator a) Which strand of DNA shown, the top or the bottom, is the template strand? b) What is the sequence of the mRNA produced from this gene? Label the 5 and 3' ends. c) What is the sequence of the protein produced from the mRNA in (b)? Label the N and C termini. If a mutation were found where a T/A (top/bottom) base pair were added immediately after the T/A base pair shown ,H 8thom in bold, what would be the sequence of the mRNA? What would be the sequence of the protein?

Explanation / Answer

c) the protein sequence is

     N- LEU STOP ARG ALA MET HIS TYR LEU ASP SER ARG LEU STOP GLU PHE ILE SER CU -C

if a mutation occured which will add a T/A ( top-botom ) base pair is added after the T/A base

the template DNA strand is

DNA:             3‘-GAT ATT TCT CGG TAC GTA AATA GAT CTA TCA TCC GAG ACTCTT AAA TAG AGT GA-5‘

mRNA:           5’- CUA UAA AGA GCCAUGCAUUUAUCUAGAUAGUAGGCUCUGAGAAUUUAUCUCACU-3’

protein:           N-LEU STOP ARG ALA MET HIS LEU SER ARG STOP STOP ALA LEU ARG ILE TYR LEU THR-C

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