What are common features between ELISA and immunofluorescence A direct and indir
ID: 186955 • Letter: W
Question
What are common features between ELISA and immunofluorescence
A
direct and indirect deterction
B antibodies
C fluorescence for detection
D Detection of antigens on the surface of cells
E use of enzymes to amplify a signal
F absorbance for detection
What is the purpose of a blocking step in ELISA
A to remove an excess of antibodies from solution
B to stop the enzymatic reaction
C to prevent nonspecific binding of an antigen and antibodies to the surface of the well
D to increase speed of the analysis
Explanation / Answer
1. Option b and d is same in both cases.
Both experimental techniques uses antibodies for detection of cell surface antigens.
2. Option c is correct.
blocking agent used for non specific and unnecessary binding of antigens to the well.
It is